| First Author | Sundnes O | Year | 2015 |
| Journal | J Invest Dermatol | Volume | 135 |
| Issue | 7 | Pages | 1771-1780 |
| PubMed ID | 25739051 | Mgi Jnum | J:222983 |
| Mgi Id | MGI:5646106 | Doi | 10.1038/jid.2015.85 |
| Citation | Sundnes O, et al. (2015) Epidermal Expression and Regulation of Interleukin-33 during Homeostasis and Inflammation: Strong Species Differences. J Invest Dermatol 135(7):1771-80 |
| abstractText | IL-33 is a novel IL-1 family member with a putative role in inflammatory skin disorders and a complex biology. Therefore, recent conflicting data regarding its function in experimental models justify a close assessment of its tissue expression and regulation. Indeed, we report here that there are strong species differences in the expression and regulation of epidermal IL-33. In murine epidermis, IL-33 behaved similar to an alarmin, being constitutively expressed in keratinocyte nuclei and rapidly lost during acute inflammation. By contrast, human and porcine IL-33 were weakly expressed or absent in keratinocytes of noninflamed skin but induced during acute inflammation. To this end, we observed that expression of IL-33 in human keratinocytes but not murine keratinocytes was strongly induced by IFN-gamma, and this upregulation completely depended on the presence of EGFR ligands. Accordingly, IFN-gamma increased the expression of IL-33 in the basal layers of the epidermis in human ex vivo skin cultures only, despite good evidence of IFN-gamma activity in cultures from both species. Together these findings demonstrate that a full understanding of IL-33 function in clinical settings must take species-specific differences into account. |
