| Primary Identifier | MGI:5476927 | Allele Type | Transgenic |
| Attribute String | Inserted expressed sequence | Gene | Tg(Hmgcr-SLAMF1)1Hrvt |
| Strain of Origin | (C57BL/6 x DBA/2) | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | A cDNA encoding human signaling lymphocytic activation molecule family member 1 (also called CD150) was inserted into the ubiquitous expression vector pHMG, which contains the promoter, untranslated first exon and first intron of the mouse 3-hydroxy-3-methylglutaryl-Coenzyme A reductase gene and the SV40 polyadenylation signal. Human SLAMF1 mRNA was detected by reverse transcription-polymerase chain reaction (RT-PCR) in multiple tissues of transgenic mice; the highest expression was observed in brain. Flow cytometry using an anti-SLAMF1 monoclonal antibody followed by FITC-conjugated goat anti-mouse antibody demonstrated the presence of human SLAMF1 on the surfaces of cells from various regions of transgenic mouse brains, including cortex, cerebellum/spinal cord, and hippocampus. |
