Primary Identifier | MGI:5477796 | Allele Type | Targeted |
Attribute String | Conditional ready, No functional change | Gene | Foxo1 |
Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
Is Recombinase | false | Is Wild Type | false |
description | ES cell line = EB3, derived from E14TG2a (129P2/OlaHsd). EB3 cells carry one inactive allele of Pou5f1. Line EB3 was created by targeted integration of E14TG2a ES cells with an IRESBSDpA selection cassette. J:195101 does not mention whether the null allele of Pou5f1 is also present in the line of mice created. |
molecularNote | A loxP site was inserted usptream of exon 2. An IRES/beta-geo cassette was fused to the coding sequence of exon 2. An additional loxP site was inserted downstream of exon 2. |