| First Author | Sanchez-Arias JC | Year | 2020 |
| Journal | eNeuro | Volume | 7 |
| Issue | 4 | PubMed ID | 32737184 |
| Mgi Jnum | J:352978 | Mgi Id | MGI:6728237 |
| Doi | 10.1523/ENEURO.0079-20.2020 | Citation | Sanchez-Arias JC, et al. (2020) Pannexin 1 Regulates Dendritic Protrusion Dynamics in Immature Cortical Neurons. eNeuro 7(4):ENEURO.0079-20.2020 |
| abstractText | The integration of neurons into networks relies on the formation of dendritic spines. These specialized structures arise from dynamic filopodia-like dendritic protrusions. It was recently reported that cortical neurons lacking the channel protein pannexin 1 (PANX1) exhibited higher dendritic spine densities. Here, we expanded on those findings to investigate, at an earlier developmental time point (with more abundant dendritic protrusions), whether differences in the properties of dendritic protrusion dynamics could contribute to this previously discovered phenomenon. Using a fluorescent membrane tag (mCherry-CD9-10) to visualize dendritic protrusions in developing neurons [at 10 d in vitro (DIV10)], we confirmed that lack of PANX1 led to higher protrusion density, while transient transfection of Panx1 led to decreased protrusion density. To quantify the impact of PANX1 expression on protrusion formation, elimination, and motility, we used live cell imaging in DIV10 neurons (one frame every 5 s for 10 min). We discovered that at DIV10, loss of PANX1 stabilized protrusions. Notably, re-expression of PANX1 in Panx1 knock-out (KO) neurons resulted in a significant increase in protrusion motility and turnover. In summary, these new data revealed that PANX1 could regulate the development of dendritic spines, in part, by controlling dendritic protrusion dynamics. |
