| First Author | Stévant I | Year | 2019 |
| Journal | Cell Rep | Volume | 26 |
| Issue | 12 | Pages | 3272-3283.e3 |
| PubMed ID | 30893600 | Mgi Jnum | J:299535 |
| Mgi Id | MGI:6488942 | Doi | 10.1016/j.celrep.2019.02.069 |
| Citation | Stevant I, et al. (2019) Dissecting Cell Lineage Specification and Sex Fate Determination in Gonadal Somatic Cells Using Single-Cell Transcriptomics. Cell Rep 26(12):3272-3283.e3 |
| abstractText | Sex determination is a unique process that allows the study of multipotent progenitors and their acquisition of sex-specific fates during differentiation of the gonad into a testis or an ovary. Using time series single-cell RNA sequencing (scRNA-seq) on ovarian Nr5a1-GFP(+) somatic cells during sex determination, we identified a single population of early progenitors giving rise to both pre-granulosa cells and potential steroidogenic precursor cells. By comparing time series single-cell RNA sequencing of XX and XY somatic cells, we provide evidence that gonadal supporting cells are specified from these early progenitors by a non-sex-specific transcriptomic program before pre-granulosa and Sertoli cells acquire their sex-specific identity. In XX and XY steroidogenic precursors, similar transcriptomic profiles underlie the acquisition of cell fate but with XX cells exhibiting a relative delay. Our data provide an important resource, at single-cell resolution, for further interrogation of the molecular and cellular basis of mammalian sex determination. |
