| Primary Identifier | MGI:5498503 | Allele Type | Transgenic |
| Attribute String | Inserted expressed sequence | Gene | Tg(Col1a1-FGF2*,-Sapphire)308Mmh |
| Strain of Origin | FVB/N | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | A transgene designed to contain the human low-molecular weight exported 18-kDa isoform of FGF2, an internal ribosome entry site (IRES), and a blue shifted variant of GFP known as Sapphire under the control of a 3.6 kb fragment of the Col1a1 5' regulatory region (Col3.6). The construct also harbors a neomycin selection cassette. Founder line 308 was established. Western blot analysis demonstrates high levels of expression of 18-kDa FGF2 in femurs and calvariae, low levels of expression in liver, and no expression in other tissues. |
