| Primary Identifier | MGI:5503122 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Nek8 |
| Transmission | Germline | Strain of Origin | (129S6/SvEvTac x C57BL/6NCrl)F1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A cassette containing an IRES-lacZ followed by an FRT flanked neomycin selection cassette was inserted in exon 3. No beta-galactosidase activity was detected in embryos. Exon 3 is spliced out of mutant transcripts and splicing of exon 2 to exon 4 causes a frameshift creating a premature stop codon. No protein is detectable by Western blot analysis. |
