| First Author | Licence S | Year | 2003 |
| Journal | Eur J Immunol | Volume | 33 |
| Issue | 4 | Pages | 1117-26 |
| PubMed ID | 12672078 | Mgi Jnum | J:199513 |
| Mgi Id | MGI:5502959 | Doi | 10.1002/eji.200323702 |
| Citation | Licence S, et al. (2003) The VpreB1 enhancer drives developmental stage-specific gene expression in vivo. Eur J Immunol 33(4):1117-26 |
| abstractText | In adult mice, the VpreB genes are expressed in bone marrow progenitor (pro-) and precursor (pre-) B cells. As part of the pre-B cell receptor, the proteins are crucial for the proliferation of these cells and consequently normal B lymphocyte development. Using cell lines, we identified a lineage- and developmental-stage-specific VpreB1 enhancer. Here, we analyze its specificity in vivo by generating transgenic mice in which expression of a reporter gene (human CD122) is regulated by the VpreB1 enhancer in the context of its own promoter. All transgenic lines expressed the reporter gene in the bone marrow in a copy number-independent manner, whereas expression levels were integration site-dependent. While the enhancer is not tissue specific, within the B cell lineage the expression pattern of human CD122 mimicked that of endogenous VpreB1. Thus, low levels were detected in pro-B cells, high levels in pre-BI and slightly lower levels in pre-BII cells; no expression was detected in immature/mature B cells. Furthermore, when in vitro cultured transgenic pre-B cells differentiated into immature B cells there was concomitant down-regulation of human CD122 and endogenous VpreB1. Thus the VpreB1 enhancer is sufficient to ensure developmental stage-specific expression of a reporter gene in B lymphocytes in vivo. |
