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Allele : Col1a1<tm13(neo/hygro*)Jae> collagen, type I, alpha 1; targeted mutation 13, Rudolf Jaenisch
Primary Identifier  MGI:5507043 Allele Type  Targeted
Attribute String  RMCE-ready Gene  Col1a1
Transmission  Cell Line Strain of Origin  (C57BL/6 x 129S4/SvJae)F1
Is Recombinase  false Is Wild Type  false
description  This allele was used to generate: Col1a1tm1(tetO/CMV-Lep)Rll, Col1a1tm1(tetO-CTNNB1)Tcd, Col1a1tm1(tetO-Cyp26b1)Mfra, Col1a1tm10(tetO-Dnmt3b_i3)Jae, Col1a1tm10(tetO*-RFP/RNAi:Rpa3)Slowe, Col1a1tm11(tetO-Dnmt3b_i6)Jae, Col1a1tm11(tetO*-RFP/RNAi:Rpa3)Slowe, Col1a1tm12(tetO-Dnmt3a_i1)Jae, Col1a1tm13(tetO-GFP/RNAi:Brd4)Slowe, Col1a1tm14(tetO-GFP/RNAi:Brd4)Slowe, Col1a1tm15(tetO-GFP/RNAi:Ren)Slowe, Col1a1tm16(tetO-GFP/RNAi:Trim33)Slowe, Col1a1tm17(tetO-GFP,-cas9*)Slowe, Col1a1tm18(tetO-GFP,-cas9*)Slowe, Col1a1tm19(tetO-GFP,-cas9*)Slowe, Col1a1tm1(CAG-Ezh2*)Meln, Col1a1tm1(CMV/tetO-Kdm2b)Atz, Col1a1tm1(Sry)Aqui, Col1a1tm1(tetO-ACMSD)Mmf, Col1a1tm1(tetO-cas9)Sho, Col1a1tm1(tetO-Ccnb1)Jvd, Col1a1tm1(tetO-CDKN2A)Ibp, Col1a1tm1(tetO-Cdx1)Ywng, Col1a1tm1(tetO-Deptor)Dmsa, Col1a1tm1(tetO-Fos)Wag, Col1a1tm1(tetO-Gata4/mCherry)Amza, Col1a1tm1(tetO-GFP/RNAi:luc)Slowe, Col1a1tm1(tetO-GFP/RNAi:Pax5)Radi, Col1a1tm1(tetO-GFP/RNAi:rluc)Beggs, Col1a1tm1(tetO-GFP/RNAi:Sirt1)Pyc, Col1a1tm1(tetO-GFP/RNAi:Stat3)Ern, Col1a1tm1(tetO-KMT2A/ELL)Dbry, Col1a1tm1(tetO-Lin28a)Gqda, Col1a1tm1(tetO-mCherry)Eggn, Col1a1tm1(tetO-Msi1)Cjl, Col1a1tm1(tetO-Pou5f1,-Klf4,-Sox2,-Myc)Hoch, Col1a1tm1(tetO-RNAi:Ptgs2,GFP)Hahe, Col1a1tm1(tetO-RNAi:Rps19)Karl, Col1a1tm1(tetO-SOX2)Mjm, Col1a1tm1(tetO-Stat1)Biat, Col1a1tm1(tetO-Tcfap2c)Hsc, Col1a1tm1(tetO-U2AF1*S34F)Mjwa, Col1a1tm1(tetO-URI1)Ndj, Col1a1tm1(tetO-YAP1*)Fcam, Col1a1tm1(tetO-Yap1*)Lrsn, Col1a1tm1(tetO-Yod1*)Hpl, Col1a1tm20(tetO-GFP,-cas9*D10A)Slowe, Col1a1tm2(CMV/tetO-Kdm2b*)Atz, Col1a1tm2(Sry)Aqui, Col1a1tm2(tetO-Ccnb2)Jvd, Col1a1tm2(tetO-Fosl2,-DsRed)Wag, Col1a1tm2(tetO-GFP/RNAi:Ren)Radi, Col1a1tm2(tetO-GFP/RNAi:Rpl5)Beggs, Col1a1tm2(tetO-GFP/RNAi:Trp53)Slowe, Col1a1tm2(tetO-LIN28B)Gqda, Col1a1tm2(tetO-RNAi:Rps19)Karl, Col1a1tm2(tetO-U2AF1)Mjwa, Col1a1tm2(tetO-URI1*S371A)Ndj, Col1a1tm2(tetO-Yap1)Lrsn, Col1a1tm3(Sry)Aqui, Col1a1tm3(tet0-Ccne1)Jvd, Col1a1tm3(tetO-Fosl1,-DsRed)Wag, Col1a1tm3(tetO-GFP/RNAi:Apc)Slowe, Col1a1tm3(tetO-Mirlet7g/Mir21)Gqda, Col1a1tm3(tetO-Pou5f1,-Sox2,-Klf4,-Myc)Jae, Col1a1tm4(Sry)Aqui, Col1a1tm4(tetO-GFP/RNAi:Apc)Slowe, Col1a1tm4(tetO-Jun/Fosl2)Wag, Col1a1tm4(tetO-Pou5f1,-Sox2,-Klf4,-Myc)Jae, Col1a1tm5(Sry)Aqui, Col1a1tm5(tetO-GFP/RNAi:Cdkn2a)Slowe, Col1a1tm5(tetO-Jun/Fos)Wag, Col1a1tm5(tetO-Pou5f1,-Klf4,-Myc)Jae, Col1a1tm6(tetO-GFP/RNAi:Cdkn2a)Slowe, Col1a1tm6(tetO-Junb/Fos,-DsRed)Wag, Col1a1tm6(tetO-MSI2)Jae, Col1a1tm7(tetO-GFP/RNAi:rluc)Slowe, Col1a1tm7(tetO-HIST1H2BJ/GFP)Jae, Col1a1tm7(tetO-Jund/Fos)Wag, Col1a1tm7(tetO-Pou5f1,-Klf4,-Sox2,-mCherry)Hoch, Col1a1tm8(tetO-GFP/RNAi:Rpa3)Slowe, Col1a1tm9(tetO-Dnmt3b_i1)Jae, Col1a1tm9(tetO-GFP/RNAi:Rpa3)Slowe, Col1a1tm1(Rr496-lacZ)Andg, Col1a1tm2(Rr492-lacZ)Andg, Col1a1tm3(Rr493-lacZ)Andg, Col1a1tm4(Rr494-lacZ)Andg, Col1a1tm5(Rr284304-lacZ)Andg, Col1a1tm6(Rr284323-lacZ)Andg.
molecularNote  ES cells containing the Gt(ROSA)26Sortm1(rtTA*M2)Jae allele were subsequently targeted to insert an FRT flanked PGK-neomycin resistance gene followed by a promoterless and ATG-less hygromycin resistance gene downstream of the Col1a1 gene. This allele can be used for RMCE.
  • mutations:
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