| Primary Identifier | MGI:5508639 | Allele Type | Transgenic |
| Attribute String | Recombinase | Gene | Tg(Phox2b-flpo)3276Grds |
| Strain of Origin | (C57BL/6 x DBA/2)F1 | Is Recombinase | true |
| Is Wild Type | false |
| molecularNote | A mouse bacterial artificial chromosome (BAC; RP24-95M11), containing the entire Phox2b (paired-like homeobox 2b) locus was obtained. This BAC also contained ~150kb sequence upstream of the Phox2b locus and ~50kb of sequence downstream of the Phox2b locus. A codon-optimized FLPo recombinase sequence fused with an SV40 polyadenylation signal, and a kanamycin resistance cassette were inserted into exon 2 of Phox2b. The resulting Phox2b::FLPo BAC transgene was injected into (C57BL/6 x DBA/2)F1 fertilized oocytes. |
