|  Help  |  About  |  Contact Us

Allele : Hand2<tm2Zllr> heart and neural crest derivatives expressed 2; targeted mutation 2, Rolf Zeller
Primary Identifier  MGI:5515377 Allele Type  Targeted
Attribute String  RMCE-ready Gene  Hand2
Transmission  Germline Strain of Origin  (129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Is Recombinase  false Is Wild Type  false
molecularNote  Double recombinase mediated cassette exchange (dRMCE) was used to replace exon 1 of the endogenous gene with a flag epitope tagged exon1. An unmodified exon 2 replaced endogenous exon 2 and a attB/attP flanked PGK-hygro selection cassette was inserted at the same time. Modification was carried out in Hand2 ES cells in which exons 1 and 2 were flanked by loxP sites and an FRT flanked neomycin cassette was inserted downstream. Replacement was accomplished by simultaneous cre and flp recombinase expression in the ES cells.
  • mutations:
  • Insertion
Paste the following link
Quick Links:
SummaryGenomePhenotypeOther
 
Quick Links:
SummaryGenomePhenotypeOther
 
Lists
This Allele isn't in any lists. Upload a list.

1 Feature

Trail: Allele

Genome

0 Expresses

0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

Other

0 Carried By

0 Driven By

4 Publication categories

Trail: Allele




MouseMine is a collaboration between MGI at The Jackson Laboratory and the InterMine project at the Cambridge Systems Biology Centre. MouseMine is funded through a grant from the NIH/NHGRI.The Jackson Laboratory makes no representation about the suitability or accuracy of this software or data for any purpose, and makes no warranties, either express or implied, including merchantability and fitness for a particular purpose or that the use of this software or data will not infringe any third party patents, copyrights, trademarks, or other rights. the software and data are provided "as is". This software and data are provided to enhance knowledge and encourage progress in the scientific community and are to be used only for research and educational purposes. Any reproduction or use for commercial purpose is prohibited without the prior express written permission of the Jackson Laboratory.

Copyright © 2017 by The Jackson Laboratory. All Rights Reserved

© 2002 - 2017 Department of Genetics, University of Cambridge, Downing Street,
Cambridge CB2 3EH, United Kingdom