| First Author | Venit T | Year | 2016 |
| Journal | Sci Rep | Volume | 6 |
| Pages | 30864 | PubMed ID | 27480647 |
| Mgi Jnum | J:356947 | Mgi Id | MGI:6222868 |
| Doi | 10.1038/srep30864 | Citation | Venit T, et al. (2016) Nuclear myosin I regulates cell membrane tension. Sci Rep 6:30864 |
| abstractText | Plasma membrane tension is an important feature that determines the cell shape and influences processes such as cell motility, spreading, endocytosis and exocytosis. Unconventional class 1 myosins are potent regulators of plasma membrane tension because they physically link the plasma membrane with adjacent cytoskeleton. We identified nuclear myosin 1 (NM1) - a putative nuclear isoform of myosin 1c (Myo1c) - as a new player in the field. Although having specific nuclear functions, NM1 localizes predominantly to the plasma membrane. Deletion of NM1 causes more than a 50% increase in the elasticity of the plasma membrane around the actin cytoskeleton as measured by atomic force microscopy. This higher elasticity of NM1 knock-out cells leads to 25% higher resistance to short-term hypotonic environment and rapid cell swelling. In contrast, overexpression of NM1 in wild type cells leads to an additional 30% reduction of their survival. We have shown that NM1 has a direct functional role in the cytoplasm as a dynamic linker between the cell membrane and the underlying cytoskeleton, regulating the degree of effective plasma membrane tension. |
