| Primary Identifier | MGI:5523157 | Allele Type | Targeted |
| Attribute String | Conditional ready, No functional change | Gene | Porcn |
| Transmission | Germline | Strain of Origin | (129S6/SvEvTac x C57BL/6NCrl)F1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | An FRT flanked neomycin cassette followed by a loxP site was inserted in intron 2 and a second loxP site was inserted just downstream of exon 3. Exon 3 is flanked by loxP sites (1154 bp). The resistance cassette was then excised from properly targeted ES cells using flp recombinase and leaving exon 3 flanked by loxP sites. Remaining FRT site 30 bp 5 prime of the 5 prime loxP site (FRT-flanked NeoR cassette excised in vitro). |
