| Primary Identifier | MGI:5544456 | Allele Type | Endonuclease-mediated |
| Gene | Rab38 | Strain of Origin | (FVB/NCrl x C57BL/6J-Rab38<cht>)F1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | TALEN-targeted homologous recombination corrected the T to G nucleotide substitution in the chocolate mutation (Rab38cht) by reconstituting the wild-type sequence encoding glycine at position 19 and thereby eliminating the exogenous SexAI restriction site (cht-GTT to wt-GGT). An identifying neutral nucleotide replacement in codon 18 (G to A) was used as a unique sequence identifier. Silent exchanges within the TALEN binding region remove an endogenous ApaLI site. This allele was identified in a line established from founder Rw2. |
