| First Author | Fischer MJ | Year | 2015 |
| Journal | PLoS One | Volume | 10 |
| Issue | 4 | Pages | e0123762 |
| PubMed ID | 25875358 | Mgi Jnum | J:233496 |
| Mgi Id | MGI:5784836 | Doi | 10.1371/journal.pone.0123762 |
| Citation | Fischer MJ, et al. (2015) Formalin evokes calcium transients from the endoplasmatic reticulum. PLoS One 10(4):e0123762 |
| abstractText | The formalin test is the most widely used behavioral screening test for analgesic compounds. The cellular mechanism of action of formaldehyde, inducing a typically biphasic pain-related behavior in rodents is addressed in this study. The chemoreceptor channel TRPA1 was suggested as primary transducer, but the high concentrations used in the formalin test elicit a similar response in TRPA1 wildtype and knockout animals. Here we show that formaldehyde evokes a dose-dependent calcium release from intracellular stores in mouse sensory neurons and primary keratinocytes as well as in non-neuronal cell lines, and independent of TRPA1. The source of calcium is the endoplasmatic reticulum and inhibition of the sarco/endoplasmic reticulum calcium-ATPase has a major contribution. This TRPA1-independent mechanism may underlie formaldehyde-induced pan-neuronal excitation and subsequent inflammation. |
