| Primary Identifier | MGI:5538399 | Allele Type | Targeted |
| Attribute String | Not Specified | Gene | Jak2 |
| Transmission | Germline | Strain of Origin | 129S2/SvPas |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP site was inserted upstream of exon 13. A lox511 site, a inverted and modified exon 13 with a point mutation (GTC to TTC) that results in the amino acid substitution of phenylalanine for valine at position 617 (V617F) and a loxP site were inserted downstream of of exon 13. Flp-mediated recombination removed the FRT-flanked neomycin resistance cassette inserted downstream of the modified exon 13 and left a lox511 site downstream of the modified exon 13. Cre-mediated recombination inverted the modified and endogenous exon 13 to allow for expression of the modified exon 13. |
