| Primary Identifier | MGI:5544427 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Cish |
| Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | An FRT- and loxP-flanked neomycin resistance cassette was inserted upstream of exon 2. An additional loxP site was inserted into exon 3 downstream of the coding sequence. Flp-mediated recombination removed the neomycin resistance cassette. Cre-mediated recombination removed the coding sequence of exon 2 and 3. Western blot analysis confirmed the absence of protein expression on CD4+ T cells. |
