| Primary Identifier | MGI:5544632 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Hand1 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| description | This allele was originally generated in ES cells heterozygous for Hand1tm1Jcc. |
| molecularNote | A cDNA containing the tethered homodimer sequence followed by a flexible linker was inserted into exon 1 downstream of the start site methionine. Cre-mediated recombination removed the floxed puromycin resistance cassette inserted downstream of exon 1. Western blot analysis confirmed the absence of monomer protein in E10.5 or E8.5 embryos. |
