| Primary Identifier | MGI:5548820 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Cfap69 |
| Transmission | Germline | Strain of Origin | C57BL/6N-A<tm1Brd> |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | KOMP-CSD |
| molecularNote | Cre-mediated excision of the parental Cfap69tm1a(KOMP)Wtsi allele resulted in the removal of the promoter-driven neomycin selection cassette and critical exon 5 leaving behind the inserted lacZ reporter sequence. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. Western blotting analysis of whole testis lysates confirmed the absence of protein expression in homozygous mutant males. |
