Primary Identifier | MGI:5558914 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Megf8 |
Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The final exon was flanked by a loxP site and an FRT-flanked neomycin resistance cassette with a 3' loxP site. Cre-mediated recombination removed the selection cassette and final exon. Western blot analysis confirmed the absence of protein expression in E12.5 embryos. |