| Primary Identifier | MGI:5556226 | Allele Type | Targeted |
| Attribute String | Conditional ready, Humanized sequence, No functional change | Gene | Tinf2 |
| Transmission | Germline | Strain of Origin | B6(Cg)-Tyr<c-2J>/J |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A targeting construct was designed to insert a loxP-flanked transcriptional STOP cassette (Puro-4x polyA) between exons 2 and 3, and an FRT-flanked neomycin (neo) resistance cassette downstream of exon 7 of the gene. A mutation was introduced in amino acid 267 in exon 6, corresponding to human amino acid 280, resulting in a lysine-to-glutamate mutation, (K267E) commonly found in patients with dyskeratosis congenital (DC). Mutant mice were bred with Tg(ACTFLPe)9205Dym mice (on a C57BL/6 congenic background (N10)) to remove the neo selection cassette. |
