| First Author | Khan I | Year | 2021 |
| Journal | Sci Rep | Volume | 11 |
| Issue | 1 | Pages | 13371 |
| PubMed ID | 34183697 | Mgi Jnum | J:359802 |
| Mgi Id | MGI:6720211 | Doi | 10.1038/s41598-021-92650-w |
| Citation | Khan I, et al. (2021) Accelerated burn wound healing with photobiomodulation therapy involves activation of endogenous latent TGF-beta1. Sci Rep 11(1):13371 |
| abstractText | The severity of tissue injury in burn wounds from associated inflammatory and immune sequelae presents a significant clinical management challenge. Among various biophysical wound management approaches, low dose biophotonics treatments, termed Photobiomodulation (PBM) therapy, has gained recent attention. One of the PBM molecular mechanisms of PBM treatments involves photoactivation of latent TGF-beta1 that is capable of promoting tissue healing and regeneration. This work examined the efficacy of PBM treatments in a full-thickness burn wound healing in C57BL/6 mice. We first optimized the PBM protocol by monitoring tissue surface temperature and histology. We noted this dynamic irradiance surface temperature-monitored PBM protocol improved burn wound healing in mice with elevated TGF-beta signaling (phospho-Smad2) and reduced inflammation-associated gene expression. Next, we investigated the roles of individual cell types involved in burn wound healing following PBM treatments and noted discrete effects on epithelieum, fibroblasts, and macrophage functions. These responses appear to be mediated via both TGF-beta dependent and independent signaling pathways. Finally, to investigate specific contributions of TGF-beta1 signaling in these PBM-burn wound healing, we utilized a chimeric TGF-beta1/beta3 knock-in (TGF-beta1(Lbeta3/Lbeta3)) mice. PBM treatments failed to activate the chimeric TGF-beta1(Lbeta3/Lbeta3) complex and failed to improve burn wound healing in these mice. These results suggest activation of endogenous latent TGF-beta1 following PBM treatments plays a key role in burn wound healing. These mechanistic insights can improve the safety and efficacy of clinical translation of PBM treatments for tissue healing and regeneration. |
