| Primary Identifier | MGI:5565335 | Allele Type | Transgenic |
| Attribute String | Recombinase | Gene | Tg(Amigo2-cre)1Sieg |
| Strain of Origin | (C57BL/6 x CBA)F2 | Is Recombinase | true |
| Is Wild Type | false |
| molecularNote | The Amigo2 gene contained in a bacterial artificial chromosome (BAC; RP23-288P18) was modified with recombineering techniques so that a cre-HSV-polyA cassette(Cre recombinase sequence with an intron in the middle of the gene to enhance mammalian expression and followed by an HSV polyadenylation signal and a portion of a neo cassette) was inserted at the translation start site. This construct was injected into B6CBAF2 pronuclei and 6 founders were obtained. When crossed to Ai14 reporter mice, CA2-specific recombinase activity was not detected in offspring of any of the crosses. With injection of an adenovirus-EYFP reporter construct into the hippocampi of adult cre animals from the 6 founder lines, one line exhibited CA2-specific reporter expression and this line was used for all subsequent experiments. |
