| First Author | Gurniak CB | Year | 2007 |
| Journal | Eur J Cell Biol | Volume | 86 |
| Issue | 1 | Pages | 3-12 |
| PubMed ID | 17049405 | Mgi Jnum | J:209786 |
| Mgi Id | MGI:5568703 | Doi | 10.1016/j.ejcb.2006.08.007 |
| Citation | Gurniak CB, et al. (2007) HuGE, a novel GFP-actin-expressing mouse line for studying cytoskeletal dynamics. Eur J Cell Biol 86(1):3-12 |
| abstractText | Analysis of actin remodeling in live cells and tissues has become an increasingly important tool to study actin-dependent cellular processes. To facilitate these experiments in the mouse we have generated a GFP-actin-expressing line (huGE) by knock-in of the GFP-actin gene into the profilin 1 locus. Here we show that GFP-actin is expressed throughout embryonic development and in all tissues except skeletal muscle, in a pattern similar to profilin 1. Particularly high expression of GFP-actin was observed in bone marrow and all blood cells. The GFP-actin fusion protein is functional as shown by its co-localization with endogenous actin in F-actin-rich structures. Therefore, the huGE mouse line provides a novel tool to monitor actin dynamics in mouse embryos and a wide range of organs. |
