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Allele : Sod2<tm1(rtTA*M2,tetO)Jsg> superoxide dismutase 2, mitochondrial; targeted mutation 1, Joel Greenberger
Primary Identifier  MGI:5568594 Allele Type  Targeted
Attribute String  Inducible, Inserted expressed sequence, Null/knockout Gene  Sod2
Transmission  Germline Strain of Origin  129S6/SvEvTac
Induced With  doxycycline/tetracycline Is Recombinase  false
Is Wild Type  false
molecularNote  The MnSOD targeting vector was designed to insert a 5.3 kbp Tet-On regulatory cassette approximately 30 nucleotides upstream of the initiation codon in the first exon. The Tet-On regulatory cassette contains the optimized form of reverse tetracycline controlled transactivator (rtTA-M2), polyA sequence, loxP site, neomycin resistance gene (neo), polyA sequence, loxP site and five copies of tetracycline operator 5' of a minimal cytomegalovirus promoter. The Tet-On regulatory fragment is a modification of the neo version of Tet-Off regulatory cassette. The Tet-Off cassette was converted to Tet-On by changing five codons via site-directed mutagenesis: S12G(GGC), E19G(GGG), A56P(CCC), D148E(GAG) and H179R(CGC). These amino acid changes converted tTA to the M2 form of rtTA (rtTA-M2).
  • mutations:
  • Insertion
  • synonyms:
  • MnSOD<tet>,
  • MnSOD<tet>
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1 Feature

Trail: Allele

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0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

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Trail: Allele




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