| Primary Identifier | MGI:5568222 | Allele Type | Targeted |
| Attribute String | Inducible, Recombinase | Gene | Hprt1 |
| Transmission | Germline | Strain of Origin | B6N.Cg-A<w-J> Hprt1<b-m3> |
| Induced With | tamoxifen | Is Recombinase | true |
| Is Wild Type | false | Project Collection | EUCOMMTOOLS |
| molecularNote | The transgene consists of a RP11-1006.F7 human bacterial artificial chromosome (BAC) retrofitted to contain a human HPRT complementary sequence (containing exon 1, intron 1, exon 2 and part of intron 2), a mouse 3' Hprt homology arm, an I-Sce linearization cut site, and a mouse 5' Hprt homology arm; all inserted into the sacB gene in the BAC vector. Next, an iCre/ERT2 fusion gene, an frt-flanked kanamycin cassette were inserted directly at the ATG of the human CRH locus. These transgene is targeted as a single copy "knock-in" into the upstream region of the Hprt |
