| Primary Identifier | MGI:5568765 | Allele Type | Transgenic |
| Attribute String | Reporter | Gene | Tg(Cspg4-EGFP*)HDbe |
| Strain of Origin | B6SJLF1 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Bacterial artificial chromosome (BAC) library (CHORI) was used to obtain a BAC (#RP23-309G21) containing the entire mouse Cspg4 (chondroitin sulfate proteoglycan 4) gene. The BAC was modified to create a membrane anchored form of EGFP (enhanced green fluorescent protein) under the control of the Cspg4 promoter followed by a polyadenylation sequence. EGFP is targeted to the plasma membrane by fusing the first 26 amino acids (containing two palmitoylation domains and a myristoylation domain) of Lck (lymphocyte-specific protein tyrosine kinase) to the EGFP N terminal. |
