| First Author | Kato T | Year | 2013 |
| Journal | Sci Rep | Volume | 3 |
| Pages | 3136 | PubMed ID | 24190364 |
| Mgi Jnum | J:207780 | Mgi Id | MGI:5559630 |
| Doi | 10.1038/srep03136 | Citation | Kato T, et al. (2013) Production of Sry knockout mouse using TALEN via oocyte injection. Sci Rep 3:3136 |
| abstractText | Recently developed transcription activator-like effector nuclease (TALEN) technology has enabled the creation of knockout mice, even for genes on the Y chromosome. In this study, we generated a knockout mouse for Sry, a sex-determining gene on the Y chromosome, using microinjection of TALEN RNA into pronuclear stage oocytes. As expected, the knockout mouse had female external and internal genitalia, a female level of blood testosterone and a female sexually dimorphic nucleus in the brain. The knockout mouse exhibited an estrous cycle and performed copulatory behavior as females, although it was infertile or had reduced fertility. A histological analysis showed that the ovary of the knockout mouse displayed a reduced number of oocytes and luteinized unruptured follicles, implying that a reduced number of ovulated oocytes is a possible reason for infertility and/or reduced fertility in the KO mouse. |
