| Primary Identifier | MGI:5571915 | Allele Type | Transgenic |
| Attribute String | Recombinase | Gene | Tg(Ltf-icre)14Mmul |
| Strain of Origin | (C57BL/6 x 129/Sv)F1 | Is Recombinase | true |
| Is Wild Type | false |
| molecularNote | A BAC (RP24-166N8) containing the Ltf gene was modified in bacterial cells to produce the targeting construct. A cassette containing codon-improved cre recombinase (icre), an artificial intron, the bovine growth hormone polyadenylation signal and an frt-flanked ampicillin selection marker was inserted into the first exon of Ltf. A plasmid endoding Flp was transfected into E. coli cells to delete the amp-marker. The BAC construct was verified by sequencing and microinjected into pronuclei of (B6/129Sv)F1 oocytes to produce transgenic animals. 2 lines (14 and 15) were obtained. Line 14 was chosen for further analysis. |
