| Primary Identifier | MGI:5583845 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Sec23b |
| Transmission | Germline | Strain of Origin | C57BL/6N |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | EUCOMM |
| molecularNote | The L1L2_Pgk_P cassette was inserted at position 144408239 of Chromosome 2 upstream of the critical exon 5 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 6 at position 144409359. The critical exons 5 and 6 are thus flanked by loxP sites. Flp recombinase expression removed the reporter and selection cassettes. Cre-mediated recombination removed exons 5 and 6. |
