| Primary Identifier | MGI:5588167 | Allele Type | Targeted |
| Attribute String | Hypomorph | Gene | Nrp1 |
| Transmission | Germline | Strain of Origin | 129S2/SvPas |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Exon 2 was replaced with a modified one in which nucleotide substitutions (TAT to GCT) result in the amino acid substitution of alanine for tyrosine at position 297 (Y297A). This mutation reduces VEGF164 binding. Cre-mediated recombination removed the floxed neomycin resistance cassette inserted downstream of the modified exon 2. Immunoblotting confirmed severely reduced protein expression in the brain, heart and kidney. |
