| Primary Identifier | MGI:5605398 | Allele Type | Targeted |
| Attribute String | Conditional ready | Gene | Ift27 |
| Transmission | Germline | Strain of Origin | C57BL/6N-A<tm1Brd> |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | EUCOMM |
| description | Cell line HEPD0653_7_E10 was successfully used to make chimeric mice. Germline transmission was accomplished. J:215449 |
| molecularNote | The L1L2_Bact_P cassette was inserted at position 78049209 of Chromosome 15 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 78051786. The critical exon(s) is/are thus flanked by loxP sites. The "conditional ready" (floxed) allele is created by flp recombinase expression in mice carrying this allele. |
