Primary Identifier | MGI:5605725 | Allele Type | Targeted |
Attribute String | Conditional ready, Reporter | Gene | Gt(ROSA)26Sor |
Transmission | Germline | Strain of Origin | (C57BL/6J x 129S6/SvEvTac)F1 |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A CAG promoter (cytomegalovirus (CMV) enhancer fused to the chicken beta-actin promoter), FRT-flanked stop cassette, loxP-flanked stop cassette, ReaChR red-shifted channelrhodopsin, fluorescent reporter mCitrine, woodchuck hepatitis post-transcriptional regulatory element (WPRE), poly A (pA) tail, and AttB/AttP-flanked neomycin resistance cassette were introduced to intron 1 of the Gt(ROSA)26Sor gene. PhiC31o-mediated recombination removed the neomycin cassette. Flp-mediated recombination removed the FRT-flanked stop cassette. |