| First Author | Tsujino K | Year | 2013 |
| Journal | Genes Cells | Volume | 18 |
| Issue | 7 | Pages | 575-88 |
| PubMed ID | 23758111 | Mgi Jnum | J:214030 |
| Mgi Id | MGI:5587885 | Doi | 10.1111/gtc.12063 |
| Citation | Tsujino K, et al. (2013) Establishment of TSH beta real-time monitoring system in mammalian photoperiodism. Genes Cells 18(7):575-88 |
| abstractText | Organisms have seasonal physiological changes in response to day length. Long-day stimulation induces thyroid-stimulating hormone beta subunit (TSHbeta) in the pars tuberalis (PT), which mediates photoperiodic reactions like day-length measurement and physiological adaptation. However, the mechanism of TSHbeta induction for day-length measurement is largely unknown. To screen candidate upstream molecules of TSHbeta, which convey light information to the PT, we generated Luciferase knock-in mice, which quantitatively report the dynamics of TSHbeta expression. We cultured brain slices containing the PT region from adult and neonatal mice and measured the bioluminescence activities from each slice over several days. A decrease in the bioluminescence activities was observed after melatonin treatment in adult and neonatal slices. These observations indicate that the experimental system possesses responsiveness of the TSHbeta expression to melatonin. Thus, we concluded that our experimental system monitors TSHbeta expression dynamics in response to external stimuli. |
