| First Author | Zizioli D | Year | 2017 |
| Journal | Eur J Cell Biol | Volume | 96 |
| Issue | 4 | Pages | 356-368 |
| PubMed ID | 28372831 | Mgi Jnum | J:347320 |
| Mgi Id | MGI:7622124 | Doi | 10.1016/j.ejcb.2017.03.008 |
| Citation | Zizioli D, et al. (2017) gamma2 and gamma1AP-1 complexes: Different essential functions and regulatory mechanisms in clathrin-dependent protein sorting. Eur J Cell Biol 96(4):356-368 |
| abstractText | gamma2 adaptin is homologous to gamma1, but is only expressed in vertebrates while gamma1 is found in all eukaryotes. We know little about gamma2 functions and their relation to gamma1. gamma1 is an adaptin of the heterotetrameric AP-1 complexes, which sort proteins in and do form clathrin-coated transport vesicles and they also regulate maturation of early endosomes. gamma1 knockout mice develop only to blastocysts and thus gamma2 does not compensate gamma1-deficiency in development. gamma2 has not been classified as a clathrin-coated vesicle adaptor protein in proteome analyses and functions for monomeric gamma2 in endosomal protein sorting have been proposed, but adaptin interaction studies suggested formation of heterotetrameric AP-1/gamma2 complexes. We detected gamma2 at the trans-Golgi network, on peripheral vesicles and identified gamma2 clathrin-coated vesicles in mice. Ubiquitous sigma1A and tissue-specific sigma1B adaptins bind gamma2 and gamma1. sigma1B knockout in mice does not effect gamma1/sigma1A AP-1 levels, but gamma2/sigma1A AP-1 levels are increased in brain and adipocytes. Also gamma2 is essential in development. In zebrafish AP-1/gamma2 and AP-1/gamma1 fulfill different, essential functions in brain and the vascular system. |
