| Primary Identifier | MGI:5635820 | Allele Type | Targeted |
| Attribute String | Inducible, Null/knockout, Recombinase | Gene | Lgr4 |
| Transmission | Germline | Strain of Origin | C57BL/6 |
| Induced With | tamoxifen | Is Recombinase | true |
| Is Wild Type | false |
| molecularNote | The targeting construct replaced exon 1 following the start codon and 287 bp of intron 1 with an mCherry reporter gene, IRES, inducible cre recombinase fusion (cre/ERT2), polyadenylation sequence and FRT-flanked neomycin resistance cassette. Flp-mediated recombination removed the selection cassette. Antibody testing and direct fluorescence failed to detect mCherry. In situ hybridization confirmed the absence of transcript expression in the intestin, skin and kidney. |
