| First Author | Abdelhamid M | Year | 2022 |
| Journal | J Alzheimers Dis | Volume | 85 |
| Issue | 4 | Pages | 1555-1571 |
| PubMed ID | 34958017 | Mgi Jnum | J:334681 |
| Mgi Id | MGI:7461991 | Doi | 10.3233/JAD-215025 |
| Citation | Abdelhamid M, et al. (2022) Probiotic Bifidobacterium breve Prevents Memory Impairment Through the Reduction of Both Amyloid-beta Production and Microglia Activation in APP Knock-In Mouse. J Alzheimers Dis 85(4):1555-1571 |
| abstractText | BACKGROUND: Probiotic supplementation reestablishes microbiome diversity and improves brain function in Alzheimer's disease (AD); their molecular mechanisms, however, have not yet been fully illustrated. OBJECTIVE: We investigated the effects of orally supplemented Bifidobacterium breve MCC1274 on cognitive function and AD-like pathologies in AppNL-G-F mice. METHODS: Three-month-old AppNL-G-F mice were orally supplemented with B. breve MCC1274 for four months. The short-term memory function was evaluated using a novel object recognition test. Amyloid plaques, amyloid-beta (Abeta) levels, Abeta fibril, amyloid-beta protein precursor and its processing enzymes, its metabolic products, glial activity, and cell proliferation in the subgranular zone of the dentate gyrus were evaluated by immunohistochemistry, Abeta ELISA, western blotting, and immunofluorescence staining. The mRNA expression levels of pro- and anti-inflammatory cytokines were determined by qRT-PCR analysis. RESULTS: We found that the oral B. breve MCC1 274 supplementation prevented memory impairment in AppNL-G-F mice and decreased hippocampal Abeta levels through the enhancement of the a-disintegrin and metalloproteinase 10 (ADAM10) level. Moreover, administration of the probiotic activated the ERK/HIF-1alpha signaling pathway responsible for increasing the ADAM10 level and also attenuated microglial activation, which in turn led to reduction in the mRNA expression levels of pro-inflammatory cytokines in the brain. In addition, B. breve MCC1274 supplementation increased the level of synaptic proteins in the hippocampus. CONCLUSION: Our findings support the possibility that oral B. breve MCC1274 supplementation might be used as a potential preventive therapy for AD progression. |
