| Primary Identifier | MGI:5689383 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Del(16Setd4-Kcnj6)12Yey |
| Transmission | Germline | Strain of Origin | 129S7/SvEvBrd-Hprt1<b-m2> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Chromosome-engineering cassettes were inserted into mouse Chromosome 16 to bracket a span between the SET domain containing 4 (Setd4) gene and the potassium inwardly-rectifying channel, subfamily J, member 6 (Kcnj6) gene. The cassette placed at the proximal locus (MICER clone MHPN267e05) was targeted upstream of Setd4 and contained a neomycin resistance gene, a loxP site, a 5' portion of an HPRT minigene, and a tyrosinase minigene. The cassette placed at the distal locus (MICER clone MHPP54c08) was targeted downstream of the Kcnj6 gene and contained an agouti transgene, a 3' portion of an HPRT minigene, a loxP site and puromycin resistance gene. Double-targeted ES cells were transiently transfected with the cre recombinase expression vector pOG231 to remove the region from Set4 to Kcnj6, which encompasses 15 genes. FISH analysis of ES cell clones confirmed the deletions. |
