Primary Identifier | MGI:5694062 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Ptk7 |
Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
Is Recombinase | false | Is Wild Type | false |
molecularNote | An FRT-flanked beta-geo cassette with a transmembrane domain and a 3' loxP site. An additional loxP site was inserted downstream of exon 5. Flp-mediated recombination removed the beta-geo cassette. Cre-mediated recombination removed exons 3 through 5 floxed. Western blot analysis confirmed the absence of protein expression in E16.5 brains. |