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Allele : Gt(ROSA)26Sor<tm1(CAG-Mir182)Dgk> gene trap ROSA 26, Philippe Soriano; targeted mutation 1, David Kirsch
Primary Identifier  MGI:5702434 Allele Type  Targeted
Attribute String  Conditional ready, Inserted expressed sequence Gene  Gt(ROSA)26Sor
Transmission  Germline Strain of Origin  129/Sv
Is Recombinase  false Is Wild Type  false
molecularNote  The targeting vector is designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), an FRT site, a loxP-flanked STOP cassette (with stop codons in all 3 reading frames and a triple polyA signal), miR-182 sequence, a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a polyA signal, and an attB/attP-flanked PGK-FRT-Neo-polyA cassette. This entire construct was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus.
  • mutations:
  • Insertion
  • synonyms:
  • LSL-miR-182,
  • LSL-miR-182
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0 Mutation Involves

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