| Primary Identifier | MGI:5702734 | Allele Type | Targeted |
| Attribute String | Humanized sequence, Inserted expressed sequence, Null/knockout, Reporter | Gene | Rho |
| Transmission | Germline | Strain of Origin | 129S7/SvEvBrd-Hprt1<b-m2> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The endogenous gene was replaced with a floxed HPRT minigene and a human RHO gene fused to GFP via homologous recombination. The human RHO gene contains a C to T nonsense mutation resulting in a premature stop codon at amino acid position 344 (Q344X). Cre mediated recombination removed the minigene. The loxP site in the 5' UTR of the human gene reduces expression about 5 fold by interfering with mRNA translation. |
