Primary Identifier | MGI:5702750 | Allele Type | Targeted |
Attribute String | Conditional ready, No functional change | Gene | Rock2 |
Transmission | Germline | Strain of Origin | 129S/SvEv or C57BL/6 |
Is Recombinase | false | Is Wild Type | false |
description | "The targeting construct was linearized and electroporated into C57BL/6 × 129/SvEv hybrid embryonic stem cells (inGenious Targeting Laboratory)." (J:222206) |
molecularNote | A single loxP site was inserted 5' to exon 3 and a loxP/FRT-flanked neomycin resistance cassette was inserted 3' to exon 3 via homologous recombination. Flp-mediated recombination deleted the neo cassette leaving exon 3 floxed. |