| Primary Identifier | MGI:5702868 | Allele Type | Targeted |
| Attribute String | Humanized sequence, Null/knockout, Reporter | Gene | Mecp2 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Nucleotide substitution(s) in exon 4 resulted in the amino acid substitution of arginine for cysteine at position 133 (R133C). This mutation affects the methyl-CpG binding domain. An EGFP was fused in-frame downstream of the coding sequence in exon 4. Cre-mediated recombination removed the floxed neomycin resistance cassette inserted downstream of EGFP. The R133C mutation represents 5% of Rhett Syndrome missense mutations. |
