| Primary Identifier | MGI:5705132 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Brpf1 |
| Transmission | Germline | Strain of Origin | C57BL/6N |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | EUCOMM |
| description | Cell line EPD0069_1_G06 was successfully used to make chimeric mice. Germline transmission was accomplished. J:222304 |
| molecularNote | The L1L2_gt2 cassette was inserted at position 113293068 of Chromosome 6 upstream of the critical exons 4 through 6 (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exons 4-6 at position 113294936. The critical exons 4 through 6 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression resulted in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. |
