| Primary Identifier | MGI:5751722 | Allele Type | Targeted |
| Gene | Gli2 | Transmission | Germline |
| Strain of Origin | 129P2/OlaHsd | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | The coding region of Gli2deltaCLR, the 3' UTR of Gli2, three tandem repeats of SV40 polyA sequence, and a floxed neomycin resistance cassette replaced 111 bp of exon 2 (the first coding exon) via homologous recombination. The Gli2deltaCLR protein fails to localize to the cilium but retains intrinsic transcriptional activity and responds to Sufu inhibition. Expression of mutant mRNA and protein is increased about 2-fold and 12-fold, respectively in homozygous embryos compared to wild-type controls. |
