| Primary Identifier | MGI:5750729 | Allele Type | Endonuclease-mediated |
| Attribute String | Endonuclease, Reporter | Gene | Gt(ROSA)26Sor |
| Strain of Origin | C57BL/6NCrl | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | The template construct used for for homology-directed repair (HDR) was designed with (from 5' to 3') a 5' homology arm, splice acceptor, polyA elements, a ubiquitously expressed CAG promoter, two copies of the bovine growth hormone poly(A) signal, loxP-flanked Neo/Stop cassette, a mammalian codon-optimized cas9 gene (derived from Streptococcus pyogenes CRISPR associated protein 9 [SpCas9]) linked to an IRES-GFP (internal ribosome entry site and Green Fluorescent Protein) reporter, and a 3' homology arm. The allele was generated by injecting the template construct, sgRosa26-1 guide RNA, and cas9 mRNA (Cas9-162A, containing plasmid coded polyadenine (polyA) tail). Cre-mediated recombination removed the floxed Neo/Stop cassette. |
