| Primary Identifier | MGI:5752394 | Allele Type | Targeted |
| Attribute String | Conditional ready, Null/knockout | Gene | Spp1 |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | 45 nucleotides in exon 2 after the start codon that encode an N-terminal signal sequence were deleted. A floxed stop and neomycin resistance cassette was inserted upstream of the modified exon 2. Cre-mediated recombination is needed to achieve expression of the intracellular isoform. Western blot analysis confirmed the absence of protein expression (intracellular or soluble isoforms) in splenocyte. |
