| Primary Identifier | MGI:5755034 | Allele Type | Targeted |
| Attribute String | Inducible, Recombinase, Reporter | Gene | Plin2 |
| Transmission | Germline | Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
| Induced With | tamoxifen | Is Recombinase | true |
| Is Wild Type | false |
| molecularNote | A promoterless mCherry reporter gene, two T2A sequence and inducible cre recombinase fusion gene (cre/ERT2) were inserted into the exon 8 in-frame upstream of the endogenous Plin2 stop codon by homologous recombination using a targeting vector encoding 6 kb 50 and 3.6 kb 30 homologous sequences. Two T2A peptide sequences separate Plin2 and mCherry and CreERT2 from each other. The targeting vector also contained a diptheria toxin A cassette for negative and a FRT-flanked PGK-neomycin cassette downstream of the mCherry-CreERT2 cassette for positive selection. Chimeric males were backcrossed onto C57BL/6J background to achieve germline transmission and bred with a mouse expressing Flp to remove the FRT-PGK-neomycin selection cassette. |
