| Primary Identifier | MGI:5756029 | Allele Type | Targeted |
| Attribute String | Conditional ready | Gene | Rasa3 |
| Transmission | Germline | Strain of Origin | C57BL/6NTac |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The HTGRS1006_A_4_H03 targeting vector was obtained from The International Knockout Mouse Consortium by The Jackson Laboratory. The L1L2_Pgk_P cassette was inserted at position 13615101 of Chromosome 8 upstream of the critical exon 3 (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 3 at position 13614300. The critical exon 3 is thus flanked by loxP sites. Flp-mediated recombination removed the reporter cassette and left exon 3 floxed. |
