| Primary Identifier | MGI:5763100 | Allele Type | Targeted |
| Gene | Gabra1 | Transmission | Germline |
| Strain of Origin | 129 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | The allele was designed to introduce a threonine-to-cysteine mutation at codon 152 (T152C counting from the start codon; or T125C in the mature peptide sequence) into exon 5 of the gene. The targeting vector also includes a loxP-flanked neomycin selection cassette upstream of exon 5, as well as a C-to-T silent mutation in the exon 5 DNA sequence (to create a HindIII site upstream of T152C for genotyping). Cre-mediated recombination removed the floxed neo cassette. |
